Truly quantitative analysis of the firefly luciferase complementation assay
نویسندگان
چکیده
منابع مشابه
Demonstration of protein-fragment complementation assay using purified firefly luciferase fragments
BACKGROUND Human interactome is predicted to contain 150,000 to 300,000 protein-protein interactions, (PPIs). Protein-fragment complementation assay (PCA) is one of the most widely used methods to detect PPI, as well as Förster resonance energy transfer (FRET). To date, successful applications of firefly luciferase (Fluc)-based PCA have been reported in vivo, in cultured cells and in cell-free ...
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ژورنال
عنوان ژورنال: Current Plant Biology
سال: 2016
ISSN: 2214-6628
DOI: 10.1016/j.cpb.2016.02.002